Introduced into the Battle for France in 1940, Matilda IIs caused problems for the Germans, whose 37mm and 50mm anti-tank guns were simply unable to penetrate the thick armour of the Matilda, famously causing consternation during the Arras counter attack. Synonymous with the ferocious battles in North Africa where it was to meet its match against the infamous 88s of the Afrika Korps, the "Queen of the Desert" nevertheless gave sterling service.

Later, as they became increasingly obsolete compared to the later German vehicles, many were sent to the Fast East where the opposing Japanese tanks were much lighter. It was the only British tank to remain in active service for the entire war.

In games of Bolt Action the Matilda is a medium tank with a light anti-tank gun that can be included in any armoured platoon.